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Table 4 Oligonucleotide primers for XynCDBFV, Xyn-MUT, and three single mutant genes

From: Improving the thermostability of a fungal GH11 xylanase via site-directed mutagenesis guided by sequence and structural analysis

Primers Primer sequences
XynCDBFV-forward 5′-CAAAGTTTCTGTAGTTCAGCTTCTC-3′
XynCDBFV-reverse 5′-ACCAATGTAAACCTTTGCGTATG-3′
Xyn-MUT-1-forward 5′-GGTGAAGCCGGTAACGTTAGCAGTGGTGCCAGTG-3′
Xyn-MUT-1-reverse 5′-CACTGCTAACGTTACCGGCTTCACCTAAAACCTTG-3′
Xyn-MUT-2-forward 5′-GGTAACGTTAGCAGTGGTTCCAGTGGTAC-3′
Xyn-MUT-2-reverse 5′- AACCACTGCTAACGTTACCGGCTTCACCTAAAAC-3′
N207S-forward 5′-TGAAGCCGGTAACGTTAGCGGTGGTGCCAGT-3′
N207S-reverse 5′-CTAACGTTACCGGCTTCACCTAAAACCTTG-3′
G208S-forward 5′-GAAGCCGGTAACGTTAACAGTGGTGCCAGT-3′
G208S-reverse 5′-TGTTAACGTTACCGGCTTCACCTAAAACCT-3′
A210S-forward 5′-GGTAACGTTAACGGTGGTTCCAGTGGTACCG-3′
A210S-reverse 5′-AACCACCGTTAACGTTACCGGCTTCACCTA-3′
  1. Nucleotide resulting in the desired mutation is underlined and in bold