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Table 4 Oligonucleotide primers for XynCDBFV, Xyn-MUT, and three single mutant genes

From: Improving the thermostability of a fungal GH11 xylanase via site-directed mutagenesis guided by sequence and structural analysis

Primers

Primer sequences

XynCDBFV-forward

5′-CAAAGTTTCTGTAGTTCAGCTTCTC-3′

XynCDBFV-reverse

5′-ACCAATGTAAACCTTTGCGTATG-3′

Xyn-MUT-1-forward

5′-GGTGAAGCCGGTAACGTTAGCAGTGGTGCCAGTG-3′

Xyn-MUT-1-reverse

5′-CACTGCTAACGTTACCGGCTTCACCTAAAACCTTG-3′

Xyn-MUT-2-forward

5′-GGTAACGTTAGCAGTGGTTCCAGTGGTAC-3′

Xyn-MUT-2-reverse

5′- AACCACTGCTAACGTTACCGGCTTCACCTAAAAC-3′

N207S-forward

5′-TGAAGCCGGTAACGTTAGCGGTGGTGCCAGT-3′

N207S-reverse

5′-CTAACGTTACCGGCTTCACCTAAAACCTTG-3′

G208S-forward

5′-GAAGCCGGTAACGTTAACAGTGGTGCCAGT-3′

G208S-reverse

5′-TGTTAACGTTACCGGCTTCACCTAAAACCT-3′

A210S-forward

5′-GGTAACGTTAACGGTGGTTCCAGTGGTACCG-3′

A210S-reverse

5′-AACCACCGTTAACGTTACCGGCTTCACCTA-3′

  1. Nucleotide resulting in the desired mutation is underlined and in bold