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Table 1 Statistics of the leaf transcriptome pre-assemblies and the final de novo assembly of A. donax

From: De novo assembly, functional annotation, and analysis of the giant reed (Arundo donax L.) leaf transcriptome provide tools for the development of a biofuel feedstock

Metric

Assembler

Final transcriptome

Trinitya

rnaSPAdesb

Trans-ABySSc

Number of sequences

136,294

138,896

158,095

62,596

Total nucleotide count (bp)

74,660,373

66,613,178

73,637,461

52,719,740

Max. transcript length (bp)

10,795

8173

10,360

10,360

Mean transcript length (bp)

547

479

465

842

N50 (bp)

659

538

520

1134

  1. Summary statistics of the A. donax pre-assemblies using Trinity, rnaSPAdes, and Trans-AbySS, and of the final A. donax leaf transcriptome after clustering and redundancy removal
  2. aTrinity: K-mer 25
  3. brnaSPAdes: K-mer 21, 33
  4. cTrans-ABySS: K-mer 17, 21, 25; the minimum transcript length was set to 200 bp