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Fig. 2 | Biotechnology for Biofuels

Fig. 2

From: Removal of the product from the culture medium strongly enhances free fatty acid production by genetically engineered Synechococcus elongatus

Fig. 2

Development of an aqueous-organic two-phase culture system. A Effects of various hydrophobic solvents on the growth of S. elongatus. WT cells were inoculated into fresh medium to give an OD730 value of 0.01 and cultivated for 72 h with 10 mL each of the organic solvents indicated. IM isopropyl myristate, De decane, Un undecane, TBP tributyl phosphate. B Effects of the two-phase culture system with IM on growth (a, b), cellular chlorophyll content (c), and the photosynthetic yield (d). WT and dAS1T cells were grown either with or without 20 mL of IM for 240 h. Data shown in a, c, and d are the mean ± SE from biological triplicates. Circles WT; triangles dAS1T. Open symbols with IM; filled symbols without IM. b shows the appearance of the cultures at t = 240 h

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