Fig. 4

TLC analysis of mannans and manno-oligosaccharides hydrolysed by mRmMan5A. mRmMan5A (5 U mL⁻¹) was incubated with 1% (w/v) mannobiose, mannotriose (a), mannotetrose (b), LBG (c), or konjac powder (d) in 50 mmol L⁻¹ McIlvaine buffer (pH 4.5) at 50 °C for 12 h. Incubation times (hours or minutes) are indicated. Lane Mn standard of manno-oligosaccharides