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Fig. 4 | Biotechnology for Biofuels

Fig. 4

From: Directed evolution of a β-mannanase from Rhizomucor miehei to improve catalytic activity in acidic and thermophilic conditions

Fig. 4

TLC analysis of mannans and manno-oligosaccharides hydrolysed by mRmMan5A. mRmMan5A (5 U mL−1) was incubated with 1% (w/v) mannobiose, mannotriose (a), mannotetrose (b), LBG (c), or konjac powder (d) in 50 mmol L−1 McIlvaine buffer (pH 4.5) at 50 °C for 12 h. Incubation times (hours or minutes) are indicated. Lane Mn standard of manno-oligosaccharides

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