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Table 1 Substrate specificity of mRmMan5A and RmMan5A

From: Directed evolution of a β-mannanase from Rhizomucor miehei to improve catalytic activity in acidic and thermophilic conditions

Substrate Specific activity (U mg−1; mean ± SD)
mRmMan5Aa RmMan5Ab
pH 4.5, 65 °C pH 4.5, 65 °C pH 7.0, 55 °C
LBG 9671.4 ± 327 3011.8 ± 53 10,756.3 ± 424
Konjac powder 5633.1 ± 102 1382.6 ± 28 5317.6 ± 172
Guar gum 1472.1 ± 51 402.9 ± 5 1492.1 ± 27
  1. aEnzymatic reactions were carried out at 65 °C for 10 min in 50 mmol L−1 McIlvaine buffer (pH 4.5)
  2. bEnzymatic reactions were carried out at 65 °C for 10 min in 50 mmol L−1 McIlvaine buffer (pH 4.5) and at 55 °C for 10 min in 50 mmol L−1 McIlvaine buffer (pH 7.0), respectively