Directed evolution of a β-mannanase from Rhizomucor miehei to improve catalytic activity in acidic and thermophilic conditions

Table 1 Substrate specificity of mRmMan5A and RmMan5A

Substrate	Specific activity (U mg⁻¹; mean ± SD)
	mRmMan5A^a	RmMan5A^b
	pH 4.5, 65 °C	pH 4.5, 65 °C	pH 7.0, 55 °C
LBG	9671.4 ± 327	3011.8 ± 53	10,756.3 ± 424
Konjac powder	5633.1 ± 102	1382.6 ± 28	5317.6 ± 172
Guar gum	1472.1 ± 51	402.9 ± 5	1492.1 ± 27

^aEnzymatic reactions were carried out at 65 °C for 10 min in 50 mmol L⁻¹ McIlvaine buffer (pH 4.5)
^bEnzymatic reactions were carried out at 65 °C for 10 min in 50 mmol L⁻¹ McIlvaine buffer (pH 4.5) and at 55 °C for 10 min in 50 mmol L⁻¹ McIlvaine buffer (pH 7.0), respectively

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