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Table 2 Kinetic parameters of mRmMan5A and RmMan5A (mean ± SD)

From: Directed evolution of a β-mannanase from Rhizomucor miehei to improve catalytic activity in acidic and thermophilic conditions

Procedure

V max (μmol min‒1 mg−1)

K m (mg mL‒1)

k cat (s‒1)

k cat/K m (mg s‒1 mL‒1)

LBG

 mRmMan5A

10,154.9 ± 293

3.27 ± 0.22

7446.9

2277.3

 RmMan5A

3192.5 ± 75

3.37 ± 0.18

2341.2

694.7

Guar gum

 mRmMan5A

1516.3 ± 47

7.06 ± 0.42

1112.0

157.5

 RmMan5A

402.9 ± 11

7.12 ± 0.39

294.8

41.4

  1. Enzymatic reactions were carried out in 50 mmol L−1 McIlvaine buffer (pH 4.5) at 65 °C for 5 min using LBG or guar gum as substrate
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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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