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Table 2 Kinetic parameters of mRmMan5A and RmMan5A (mean ± SD)

From: Directed evolution of a β-mannanase from Rhizomucor miehei to improve catalytic activity in acidic and thermophilic conditions

Procedure V max (μmol min‒1 mg−1) K m (mg mL‒1) k cat (s‒1) k cat/K m (mg s‒1 mL‒1)
LBG
 mRmMan5A 10,154.9 ± 293 3.27 ± 0.22 7446.9 2277.3
 RmMan5A 3192.5 ± 75 3.37 ± 0.18 2341.2 694.7
Guar gum
 mRmMan5A 1516.3 ± 47 7.06 ± 0.42 1112.0 157.5
 RmMan5A 402.9 ± 11 7.12 ± 0.39 294.8 41.4
  1. Enzymatic reactions were carried out in 50 mmol L−1 McIlvaine buffer (pH 4.5) at 65 °C for 5 min using LBG or guar gum as substrate