Proximity ligation scaffolding and comparison of two Trichoderma reesei strains genomes

Table 3 Translocation and large deletion events found in GRAAL reassembly of T. reesei Rut-C30 with respect to QM6a

Translocation	Location on scaffolds (this study)	Location on scaffolds [15]	Mapping on QM6a chromosomes
n° 1	scaffold_2: 556 ± 22 kb	scaffold_2: 546,703 bp	chr III: 3,166,447
n° 1	scaffold_4: 1,197 ± 25 kb	scaffold_4: 1,204,862 bp	chr I: 4,342,096
n° 2	scaffold_4: 750 ± 27 kb	scaffold_4: 748,277 bp	chr I: 3,885,511
n° 2	scaffold_22: 138 ± 31 kb	scaffold_22: 139,515 bp	chr VI: 3,165,364
n° 3	scaffold_22: 138 ± 31 kb	scaffold_22: 139,476 bp	chr VI: 3,165,325
n° 3	scaffold_48: 0 ± 35 kb	scaffold_48: 1667 bp	chr I: 5,018,020

Large deletion	Location on scaffold (this study)	Location on scaffold [14]	Mapping on QM6a chromosome
85-kb deletion	scaffold_15: 0–85 ± 25 kb	scaffold_15: 1,555–86,603	chr VI: 52,198–137,246

Newly acquired 3C-seq data of T. reesei Rut-C30 strain were used to reassemble the reference genome. Comparison with QM6a reassembly allowed the identification of three chromosomal translocations and one large deletion. The position of these rearrangements is consistent with former work [14, 15]

ISSN: 2731-3654