Fig. 2

Effect of the carbon source and glucose addition on BGLU production. a BGLU production in different soluble carbon sources. The fungus was grown in Mandels with 1% glucose (triangles), glycerol (crosses), fructose (asterisks), or cellobiose (squares) for 7 days. Samples were taken daily and BGLU activity was measured in the supernatants. b Glucose consumption measured in Mandels + 1% glucose cultures. Samples were taken daily and glucose concentration of supernatants was measured by HPLC as described in “Methods”. c Effect of glucose addition on BGLU production. To study the influence of glucose, 0.5 or 1% glucose pulse feeds were daily added to 1% glucose or Avicel cultures. Arrows indicate the addition of the monosaccharide. Samples were taken daily and BGLU activity measured in the supernatants