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Table 1 Enzymatic assays conditions

From: Management of enzyme diversity in high-performance cellulolytic cocktails

Activity Substrate Product reference Substrate concentration (g/l) Temp. (°C) pH Time (min) References
Beta-glucosidase pNPGa Sigma N7006 0.1 50 5.0 10 [40]
Beta-xylosidase pNXPb Sigma 487870 0.1 50 5.0 10 [40]
Cellobiohydrolase Avicel Sigma 11365 10.0 50 5.0 120 [41, 42]
Endoglucanase Azo-CMCc Sigma 18693 10.0 50 5.0 10 [43]
Endoxylanase Azo-WAXd Megazyme 10.0 50 5.0 10 [44]
  1. a p-Nitrophenyl beta-d-glucopyranoside
  2. b p-Nitrophenyl-beta-d-xylopyranoside
  3. cAzo-carboxymethyl cellulose
  4. dAzo-wheat arabinoxylan