Table 1 Enzymatic assays conditions
From: Management of enzyme diversity in high-performance cellulolytic cocktails
Activity | Substrate | Product reference | Substrate concentration (g/l) | Temp. (°C) | pH | Time (min) | References |
|---|---|---|---|---|---|---|---|
Beta-glucosidase | pNPGa | Sigma N7006 | 0.1 | 50 | 5.0 | 10 | [40] |
Beta-xylosidase | pNXPb | Sigma 487870 | 0.1 | 50 | 5.0 | 10 | [40] |
Cellobiohydrolase | Avicel | Sigma 11365 | 10.0 | 50 | 5.0 | 120 | |
Endoglucanase | Azo-CMCc | Sigma 18693 | 10.0 | 50 | 5.0 | 10 | [43] |
Endoxylanase | Azo-WAXd | Megazyme | 10.0 | 50 | 5.0 | 10 | [44] |