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Fig. 2 | Biotechnology for Biofuels

Fig. 2

From: The LacI family protein GlyR3 co-regulates the celC operon and manB in Clostridium thermocellum

Fig. 2

Protein–DNA interactions at potential GlyR3 binding sites near celC, manB, and celT genes. Electrophoretic Mobility Shift Assay was used to assess binding of GlyR3 to putative DNA binding sites for a celC, b manB, c celT, and d a random sequence of DNA. Figure shows GlyR3 binding to the DNA sites near celC and manB but no binding to the site near celT or to random DNA. a Lane 1 celC; Lane 2 celC + GlyR3; Lane 3 celC + GlyR3 + unlabeled 18-mer. b Lane 1 manB; Lane 2 manB + GlyR3; Lane 3 manB + GlyR3 + unlabeled 18-mer. c Lane 1 celT; Lane 2 celT + GlyR3; Lane 3 celT + GlyR3 + unlabeled 18-mer. d Lane 1 random DNA; Lane 2 DNA + GlyR3; Lane 3 DNA + GlyR3 + unlabeled 18-mer. In all cases, the target DNA is 0.2 ng (celC = 0.156 nM, manB = 0.187 nM, celT = 0.196 nM, control = 0.204 nM), GlyR3 is 150 ng (387 nM), and 18-mer competitive DNA is 500 ng (2.14 mM)

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