Fig. 2From: The LacI family protein GlyR3 co-regulates the celC operon and manB in Clostridium thermocellum Protein–DNA interactions at potential GlyR3 binding sites near celC, manB, and celT genes. Electrophoretic Mobility Shift Assay was used to assess binding of GlyR3 to putative DNA binding sites for a celC, b manB, c celT, and d a random sequence of DNA. Figure shows GlyR3 binding to the DNA sites near celC and manB but no binding to the site near celT or to random DNA. a Lane 1 celC; Lane 2 celC + GlyR3; Lane 3 celC + GlyR3 + unlabeled 18-mer. b Lane 1 manB; Lane 2 manB + GlyR3; Lane 3 manB + GlyR3 + unlabeled 18-mer. c Lane 1 celT; Lane 2 celT + GlyR3; Lane 3 celT + GlyR3 + unlabeled 18-mer. d Lane 1 random DNA; Lane 2 DNA + GlyR3; Lane 3 DNA + GlyR3 + unlabeled 18-mer. In all cases, the target DNA is 0.2 ng (celC = 0.156 nM, manB = 0.187 nM, celT = 0.196 nM, control = 0.204 nM), GlyR3 is 150 ng (387 nM), and 18-mer competitive DNA is 500 ng (2.14 mM)Back to article page