Fig. 3
From: The LacI family protein GlyR3 co-regulates the celC operon and manB in Clostridium thermocellum
Relative strength of celC and manB GlyR3 binding stites. EMSA of celC and manB binding sites as a function of GlyR3 level. The assay reveals that GlyR3 binds more readily to the binding site in celC compared to the binding site in manB. DNA loading was 0.2 ng (celC: 0.156 nM, manB: 0.187 nM). GlyR3 loadings were: 140 ng (361 nM), 60 ng (155 nM), 50 ng (129 nM), 40 ng (103 nM), 30 ng (77 nM), 15 ng (39 nM), and 10 ng (26 nM)