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Fig. 4 | Biotechnology for Biofuels

Fig. 4

From: The LacI family protein GlyR3 co-regulates the celC operon and manB in Clostridium thermocellum

Fig. 4

Effect of laminaribiose on interaction of GlyR3 with DNA. EMSA was used to determine the effect of laminaribiose addition on GlyR3 binding to DNA binding sites. Laminaribiose disrupted the binding of GlyR3 to the celC binding site but had no measurable effect on interactions between GlyR3 and the manB binding site. Lane 1 celC [0.2 ng (0.156 nM)]; Lane 2 celC + GlyR3 [150 ng (387 nM)]; Lane 3 celC + GlyR3 + laminaribiose [35 μg (50 mM)]; Lane 4 celC + GlyR3 + laminaribiose [70 μg (100 mM)]; Lane 5 manB [0.2 ng (0.187 nM)]; Lane 6 manB + GlyR3[150 ng (387 nM)]; Lane 7 manB + GlyR3 + laminaribiose [35 μg (50 mM)]; Lane 8 manB + GlyR3 + laminaribiose [70 μg (100 mM)]

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