Fig. 3

Quantitative assessment of transient EYFP gene expression as directed by SCBV21, Ubi1, Pr4, CaMV 35S or enhanced CaMV 35S (2×35S) in sugarcane young leaf segments. Values of a EYFP focus count and b total EYFP expression level (10⁴) (mean gray value × pixels) were collected from representative images monitored with a stereomicroscope (Olympus SZX7, Olympus) fitted with YFPHQ filters (excitation of 490–500 nm and emission of 515–560 nm) and a DP71 digital camera (Olympus) (×15 magnification) at 48 h post-DNA bombardment and calculated using ImageJ software as described in “Methods”. Values represent means with standard error from three independent experiments and nine replicates per experiment. Means with the same letter are not significantly different at p > 0.05