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Table 2 The SCBV21 promoter drives expression of the GUS gene preferentially in the sugarcane culm and leaf

From: A novel Sugarcane bacilliform virus promoter confers gene expression preferentially in the vascular bundle and storage parenchyma of the sugarcane culm

Transgenic line GUS copy number GUS activity (pmol of 4-methylumbelliferone/min/µg protein)a
Culm Leaf Root
SCBV21:GUS 8–14 2649.1 ± 41.3 (2466.3–3252.1) 1260.0 ± 201.8 (1127.9–1384.8) 165.3 ± 4.8 (126.0–233.9)
Ubi1:GUS 13–19 23.6 ± 1.5 (18.3–42.5) 46.6 ± 2.6 (31.6–57.4) 58.1 ± 9.0 (37.1–80.1)
35S:GUS 4–23 3.8 ± 0.9 (2.0–5.4) 1.3 ± 0.2 (0.4–3.0) 7.2 ± 0.3 (5.5–9.0)
Nontransformed   8.8 ± 0.5 (0.5–13.0) 0.5 ± 0.03 (0.05–0.1) 3.9 ± 0.1 (3.6–4.3)
  1. aAverage GUS activity was measured in culms, leaves and roots of 1 year-old sugarcane transgenic for SCBV21:GUS. Ubi1:GUS and 35S:GUS lines were included as a positive control. The number of independent SCBV21:GUS, Ubi1:GUS and 35S:GUS transgenic lines tested were 5, 5 and 7, respectively. GUS activity represents three technical repetitions and is reported with the standard error. The range of GUS activities for each set of experiments is indicated in parentheses