A novel Sugarcane bacilliform virus promoter confers gene expression preferentially in the vascular bundle and storage parenchyma of the sugarcane culm

Table 3 The SCBV21 promoter drives high levels of GUS gene expression in the sugarcane culm

SCBV21:GUS transgenic line	GUS activity (pmol of 4-methylumbelliferone/min/µg protein)^a
SCBV21:GUS transgenic line	Top	Middle	Bottom
5A (CP84-1198)	2648.8 ± 27.5	2535.4 ± 48.6	2466. 3 ± 59.3
3501 (CP72-1210)	2560.0 ± 49.4	2500.8 ± 40.4	2574.8 ± 34.6
3512 (CP72-1210)	2668.5 ± 13.1	2668.5 ± 9.9	2688.3 ± 58.9
3515 (CP72-1210)	2653.7 ± 39.5	2569.9 ± 74.6	3252.1 ± 42.2
35123 (CP72-1210)	2693.2 ± 21.5	2651.3 ± 65.3	2604.4 ± 34.6
Nontransformed (CP72-1210)	6.7 ± 0.5	10.4 ± 0.6	9.2 ± 0.4

^aAverage GUS activity was measured in culm top, middle and bottom sections of 1-year-old sugarcane transgenic for SCBV21:GUS. The number of independent SCBV21:GUS transgenic lines tested was five (The range of copy number of GUS in these lines is 8–14). GUS activity represents six technical repetitions and is reported with the standard error

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