Fig. 2

Ethyl acetate biosynthetic pathways and synthesis activities in K. marxianus. a Ethyl acetate biosynthesis via alcohol-O-acetyltransferase (Atf; left), reverse esterase activity (middle), and by alcohol dehydrogenase (Adh) oxidation of hemiacetal (right). b K. marxianus CBS 6556 lysate assays for ethyl acetate production. Reactions were accomplished with 100 µg of lysate protein buffered to pH 7.2 with 100 mM potassium phosphate and 30 °C. Data points represent the arithmetic mean of three biological replicates, and the error bars represent the standard deviation