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Fig. 5 | Biotechnology for Biofuels

Fig. 5

From: CRISPR–Cas9-enabled genetic disruptions for understanding ethanol and ethyl acetate biosynthesis in Kluyveromyces marxianus

Fig. 5

Transcriptional analysis of KmADH expression in wild-type K. marxianus CBS 6556. mRNA copy numbers at lag, log, and stationary phases of aerobic (a) and anaerobic (b) growth were determined by RT-qPCR. The top panels of a and b show the total mRNA copy numbers of KmADH1–7 expressed in K. marxianus strain CBS 6556 at 5 h for aerobic cultures and 6 h for anaerobic cultures. mRNA copy number represents total number of transcripts for 5 ng of isolated RNA. The heat maps below show the mRNA copy fold change of the individual genes at log (10 and 14 h) and stationary phases (18 and 24 h) compared with the expression at lag phase (5 and 6 h). Bars and error bars represent the arithmetic mean and standard deviation of triplicate biological samples

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