CRISPR–Cas9-enabled genetic disruptions for understanding ethanol and ethyl acetate biosynthesis in Kluyveromyces marxianus

Table 1 Kluyveromyces marxianus alcohol dehydrogenases (Adh) and alcohol-O-acetyltransferase (Atf)

Name	Alternative names	Putative function	Homology (identity, similarity)	Size	Reference	Gene product
KmAdh1	–	Ethanol production	KlAdh1 (85.2, 92.0%) KlAdh2 (86.5, 92.0%) ScAdh2 (79.4, 88.0%) ScAdh1 (76.3, 84.8%)	348aa	[19,20,21,22,23,24]	BAO40126.1
KmAdh2	–	Ethanol production	ScAdh1 (86.0, 91.4%) KlAdh2 (83.7, 91.1%) KlAdh1 (86.0, 90.9%) ScAdh2 (84.5, 90.3%)	348aa	[19,20,21,22,23,24]	BAO40244.1
KmAdh3	–	Use of nonfermentable carbon sources	KlAdh3 (91.2, 94.4%) ScAdh3 (77.5, 87.0%)	375aa	[19,20,21,22,23,24]	BAO42617.1
KmAdh4	ADH4b [19]	Ethanol detoxification	KlAdh4 (90.8, 93.9%) ScAdh3 (78.9, 87.6%)	379aa	[19,20,21,22,23,24]	BAO38616.1
KmAdh5	ADHb [20], ADH4a [19]	Unknown	ScAdh3 (65.2, 78.3%)	418aa	[19, 20, 23]	BAO38463.1
KmAdh6		Unknown	ScAdh6 (62.0, 76.4%) ScAdh7 (58.8, 74.3%)	366aa	[19, 20, 23]	BAO42650.1
KmAdh7	Adha [20], adh [19]	Unknown	Acinetobacter equi adh (67.2, 81.4%) Snodgrassella alvi adh (67.4, 80.9%) Cupriavidus necator adh (30.8, 50.2%)	386aa	[19, 20]	BAO40648.1
KmAtf	Atf1 [19]	Unknown	KlAtf (56.0, 75.8%) ScAtf2 (31.7, 53.1%) ScAtf1 (35.0, 52.5%)	515aa	[19, 20]	BAO39498.1

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