Table 1 Comparison of fermentation parameters of different strains in shake flask cultivation
From: A new genome-scale metabolic model of Corynebacterium glutamicum and its application
Strain | μ max (1/h) | DCW (g/L) | l-Alanine (g/L) | l-Valine (g/L) | Citrate (g/L) | α-Oxoglutarate (g/L) | Succinate (g/L) | l-Proline (g/L) | Yieldpro (mol/mol) |
|---|---|---|---|---|---|---|---|---|---|
WT | 0.27 ± 0.01 | 18.62 ± 0.02 | 4.98 ± 0.07 | 1.49 ± 0.24 | 6.88 ± 0.37 | 1.05 ± 0.14 | 8.01 ± 0.62 | – | – |
Pro-1 (WTproB*) | 0.26 ± 0.00 | 17.60 ± 0.51 | 4.85 ± 0.07 | 1.37 ± 0.27 | 7.02 ± 0.56 | 1.08 ± 0.09 | 7.18 ± 0.80 | 0.88 ± 0.12 | 0.03 ± 0.00 |
Pro-2 (WTproB*∆putA) | 0.25 ± 0.00 | 17.20 ± 0.46 | 4.72 ± 0.07 | 1.36 ± 0.28 | 7.54 ± 0.23 | 1.47 ± 0.20 | 7.44 ± 0.82 | 1.69 ± 0.10 | 0.05 ± 0.00 |
Pro-3 (WTproB*P pck ::P acn acn*∆putA) | 0.26 ± 0.00 | 16.45 ± 0.13 | 4.54 ± 0.11 | 1.36 ± 0.37 | 5.48 ± 0.32 | 1.82 ± 0.29 | 7.25 ± 0.91 | 1.79 ± 0.02 | 0.05 ± 0.00 |
Pro-4 (WTproB*P glyA ::P acn acn*∆putA) | 0.25 ± 0.00 | 16.27 ± 0.12 | 4.42 ± 0.04 | 1.30 ± 0.50 | 4.98 ± 0.16 | 2.05 ± 0.32 | 7.33 ± 0.86 | 2.01 ± 0.07 | 0.06 ± 0.00 |
Pro-5 (WTproB*P eftu ::P acn acn*∆putA) | 0.23 ± 0.00 | 15.94 ± 0.10 | 3.92 ± 0.10 | 1.25 ± 0.10 | 2.01 ± 0.28 | 2.56 ± 0.09 | 8.19 ± 0.57 | 2.26 ± 0.03 | 0.07 ± 0.00 |
Pro-6 (WTproB*P eftu ::P acn acn*∆putA/pXMJ19-proB*) | 0.20 ± 0.00 | 14.65 ± 0.07 | 2.12 ± 0.13 | 0.72 ± 0.05 | 1.63 ± 0.27 | 0.37 ± 0.01 | 4.47 ± 0.85 | 18.71 ± 0.21 | 0.56 ± 0.00 |