Fig. 3
From: Metabolic engineering of Escherichia coli for production of n-butanol from crude glycerol
Microaerobic production of n-butanol in the strain with the enhanced glycerol catabolism. Escherichia coli strain BuT-12-2 was engineered by enhancement of the gldA-dhaKLM catabolic route. The strain was grown in M9Y medium containing 20 g/L pure glycerol and the fermentations were carried out for 24 h. The experiments were conducted in triplicate