Fig. 4From: Metabolic engineering of Escherichia coli for production of n-butanol from crude glycerolMicroaerobic production of n-butanol in the strain with the gluconeogenic flux via the reductive PP pathway. Escherichia coli strain BuT-12-3 was engineered by the removal of zwf. The strain was grown in M9Y medium containing 20 g/L pure glycerol and the fermentations were carried out for 24 h. The experiments were conducted in triplicateBack to article page