|
Strain
|
P
B
|
Y
B/G
|
Gene product targeted for manipulation
|
|---|
|
PDH
|
Zwf
|
Pgl
|
GldA
|
DhaKLM
|
GltA
|
|---|
|
BuT-8
|
0.054
|
0.16
|
W
|
W
|
W
|
W
|
W
|
W
|
|
BuT-12A
|
0.09
|
0.23
|
+
|
+
|
+
|
W
|
W
|
W
|
|
BuT-12-2
|
0.13
|
0.29
|
+
|
+
|
+
|
+
|
+
|
+
|
|
BuT-12-3
|
0.14
|
0.32
|
+
|
−
|
+
|
+
|
+
|
W
|
|
BuT-16
|
0.18
|
0.35
|
+
|
+
|
+
|
+
|
+
|
<
|
| |
0.24*
|
0.34*
| | | | | | |
- The fermentation was carried out with the cell density at OD550 of 0.2. Strain BuT-16 was grown in M9Y medium containing crude glycerol of 20 g/L for 40 h. The others were cultured on pure glycerol of 20 g/L for 24 h. The development course of producer strains for the production of n-butanol was shown in Additional file 1: Fig. S1. Note: W, wild type; +, enhancement; −, absence; <, suppression; P
B, n-butanol productivity (g/L/h); Y
B/G, conversion yield of n-butanol on glycerol (g/g)
- * The fermentation was conducted with the cell density at OD550 of 5 and crude glycerol of 30 g/L for 36 h
