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Table 2 Summary of the fermentation kinetics for producer strains

From: Metabolic engineering of Escherichia coli for production of n-butanol from crude glycerol

Strain

P B

Y B/G

Gene product targeted for manipulation

PDH

Zwf

Pgl

GldA

DhaKLM

GltA

BuT-8

0.054

0.16

W

W

W

W

W

W

BuT-12A

0.09

0.23

+

+

+

W

W

W

BuT-12-2

0.13

0.29

+

+

+

+

+

+

BuT-12-3

0.14

0.32

+

+

+

+

W

BuT-16

0.18

0.35

+

+

+

+

+

<

 

0.24*

0.34*

      
  1. The fermentation was carried out with the cell density at OD550 of 0.2. Strain BuT-16 was grown in M9Y medium containing crude glycerol of 20 g/L for 40 h. The others were cultured on pure glycerol of 20 g/L for 24 h. The development course of producer strains for the production of n-butanol was shown in Additional file 1: Fig. S1. Note: W, wild type; +, enhancement; −, absence; <, suppression; P B, n-butanol productivity (g/L/h); Y B/G, conversion yield of n-butanol on glycerol (g/g)
  2. * The fermentation was conducted with the cell density at OD550 of 5 and crude glycerol of 30 g/L for 36 h