Fig. 8

Heterologous expression of NoDGAT1A enhanced TAG synthesis in the C. reinhardtii strain UVM4. a NoDGAT1A mRNA levels (normalized to endogenous β-actin gene) in NoDGAT1A heterologous expression lines and EV, as determined by quantitative real-time PCR. b TAG contents in NoDGAT1A heterologous expression lines and EV. c, d Relative abundance of fatty acids in the sn-1/sn-3 (c) and sn-2 (d) positions of TAG in NoDGAT1A heterologous expression lines and EV under nitrogen-depleted conditions (24 h). Algal cells grown in nitrogen-replete medium for 4 days (considered as 0 h of nitrogen depletion) were used for nitrogen-depletion experiment. Data in (a–d) are expressed as mean ± SD (n = 3). Asterisks indicate the significant difference compared with EV (t-test, P < 0.05)