Fig. 6

Saccharification of pretreated Norway spruce in the presence (black bars) or absence (white bars) of 1 mM ascorbic acid. Enzymatic hydrolysis was carried out with the Celluclast^®:Novozym 188 mix (5: 1 w/w) supplemented with major Trichoderma reesei cellulases (TrCel7A, TrCel6A, and TrCel7B) or TaLPMO9A. The total protein loading was 8 mg/g glucan, except for the sample labeled “85%,” where the total protein loading was at 6.8 mg/g glucan. The indicated individual enzymes comprised 15% of the total protein loading; BSA was used as a control. Experiments were carried out in reactions containing 5% DM in 50 mM sodium acetate pH 5.0 at 50 °C and were incubated at 50 °C for 48 h. The data points represent the average value of three independent experiments with one technical replicate per experiment. The error bars represent standard deviations of the three independent experiments. The statistical significance of differences in glucan conversion between the control reactions with BSA and reactions with added individual enzymes (in the presence or absence of 1 mM ascorbic acid) was analyzed using two-way ANOVA with Tukey’s post hoc test (95% confidence interval) and is indicated as follows: *p < 0.05, **p < 0.01, ***p < 0.001