Fig. 3
Western blots for the detection of AdhE2 via the C-terminal 6X His tag: a in both E. coli and R. palustris. Expected AdhEBisB18, AdhE2824, and AdhE2opti sizes are 94.7, 94.3, and 94.3 kD, respectively (outlined with dotted box). Downfield band of ~50 kD (outlined with dotted box) likely corresponds to the fragmented C-terminal alcohol dehydrogenase domain where the 6X His tag is located. Protein amounts of 135 μg were loaded into all sample lanes. The red band in the R. palustris anaerobic lane is from photo pigments and may partially be obscuring signal from the western blot: b in R. palustris pBBR1MCS-2 lacp adhE2 opti cultures. The signal size of aerobic cultures of R. palustris pBBR1MCS-2 lacp adhE2 opti corresponded with the expected size of AdhE2opti (94.3 kD)