Table 1 Comparison of different glycosyl residue analysis methods for μg monosaccharide quantified per mg of AIR from four different leaf samples

Arabidopsis

 Alditol acetate

21.8 ± 0.8

13.3 ± 0.7

3.5 ± 0.3

23.9 ± 1.3

–

–

5.2 ± 0.3

38.6 ± 2.3

14.0 ± 0.6

 Uronic acid*

–

–

–

–

108.6 ± 3.2

–

–

–

 Alditol acetate + uronic acid**

21.8 ± 0.8^a

13.3 ± 0.7^a

3.5 ± 0.3^b

23.9 ± 1.3^a

108.6 ± 3.2

5.2 ± 0.3^b

38.6 ± 2.3^a

14.0 ± 0.6^a

 Carbodiimide

27.4 ± 1.8^b

23.5 ± 1.5^c

2.7 ± 0.02^a

32.7 ± 1.4^b

1.7 ± 0.01

64.8 ± 4.1^b

4.8 ± 0.3^a

46.6 ± 2.1^c

26.1 ± 1.3^b

 TMS

29.2 ± 2.2^c

22.1 ± 1.9^b

3.5 ± 0.03^b

32.2 ± 1.1^b

1.9 ± 0.02

72.5 ± 3.3^b

5.2 ± 0.5^a

42.3 ± 2.6^b

24.3 ± 2.2^b

 HPAEC

27.4 ± 1.4^b

20.0 ± 1.0^b

3.3 ± 0.02^b

35.5 ± 1.5^c

1.8 ± 0.01

62.4 ± 3.9^a

8.9 ± 0.7^c

41.0 ± 2.8^b

23.4 ± 1.6^b

Populus

 Alditol acetate

55.2 ± 3.8

13.6 ± 0.7

5.3 ± 0.2

41.5 ± 2.6

–

–

3.9 ± 0.4

35.7 ± 2.5

20.5 ± 0.8

 Uronic acid*

–

–

–

–

91.4 ± 4.1

–

–

–

 Alditol acetate + uronic acid**

55.2 ± 3.8^a

13.6 ± 0.7

5.36 ± 0.2

41.5 ± 2.6^a

91.4 ± 4.1

3.9 ± 0.4^a

35.7 ± 2.5^b

20.5 ± 0.8

 Carbodiimide

98.1 ± 5.1^b

11.2 ± 0.7

5.1 ± 0.2

51.5 ± 2.9^b

0.9 ± 0.01

29.5 ± 3.1^b

3.6 ± 0.3^a

44.6 ± 2.9^c

21.2 ± 1.3

 TMS

99.2 ± 4.9^b

12.4 ± 0.9

5.9 ± 0.4

54.1 ± 3.2^b

1.0 ± 0.01

32.8 ± 2.9^b

5.1 ± 0.4^b

42.3 ± 2.1^c

20.2 ± 1.8

 HPAEC

95.7 ± 6.3^b

10.6 ± 0.8

4.2 ± 0.3

60.3 ± 4.1^c

0.7 ± 0.01

25.6 ± 2.6^a

8.6 ± 0.6^c

34.3 ± 1.8^a

19.7 ± 1.4

Rice

 Alditol acetate

64.1 ± 3.9

2.8 ± 0.1

0.7 ± 0.02

220.3 ± 8.9

 -

 -

1.3 ± 0.01

16.5 ± 1.1

31.1 ± 2.4

 Uronic acid*

 -

 -

 -

 -

12.1 ± 0.9

 -

 -

 -

 Alditol acetate + uronic acid**

64.1 ± 3.9^a

2.8 ± 0.1

0.7 ± 0.02

220.3 ± 8.9^b

12.1 ± 0.9

1.3 ± 0.01^a

16.5 ± 1.1^b

31.1 ± 2.4^b

 Carbodiimide

70.3 ± 3.6^b

3.1 ± 0.3

0.5 ± 0.01

212.3 ± 9.3^a

1.1 ± 0.20

4.3 ± 0.4^b

1.2 ± 0.02^a

11.6 ± 0.8^a

34.3 ± 2.8^b

 TMS

73.4 ± 4.2^b

2.8 ± 0.3

0.4 ± 0.01

214.5 ± 11.3^a

1.2 ± 0.02

4.9 ± 0.4^b

2.0 ± 0.01^a

11.4 ± 0.9^a

33.5 ± 1.9^b

 HPAEC

75.3 ± 5.8^c

2.9 ± 0.2

0.8 ± 0.02

228.9 ± 14.3^c

0.6 ± 0.01

3.6 ± 0.3^a

3.1 ± 0.04^b

10.8 ± 0.6^a

25.6 ± 2.1^a

Switchgrass

 Alditol acetate

71.3 ± 2.4

2.5 ± 0.3

1.0 ± 0.02

281.0 ± 13.3

 -

 -

1.4 ± 0.02

15.8 ± 1.6

50.2 ± 4.1

 Uronic acid*

 -

 -

 -

 -

4.5 ± 0.4

 -

-  

 -

 Alditol acetate + uronic acid**

71.3 ± 2.4^a

2.5 ± 0.3

1.0 ± 0.02^b

281.0 ± 13.3^a

4.5 ± 0.4

1.4 ± 0.02^a

15.8 ± 1.6^c

50.2 ± 4.1^c

 Carbodiimide

75.8 ± 3.3^a

2.5 ± 0.3

0.6 ± 0.01^a

290.8 ± 12.7^b

0.8 ± 0.01

3.6 ± 0.2^a

2.0 ± 0.02^b

12.6 ± 0.7^b

43.7 ± 3.9^b

 TMS

80.3 ± 5.1^b

2.7 ± 0.4

0.8 ± 0.01^b

295.3 ±  ± 13.8^b

0.9 ± 0.01

4.1 ± 0.4^b

1.0 ± 0.01^a

11.9 ± 1.0^b

45.3 ± 3.5^b

 HPAEC

84.0 ±  ± 3.7^b

2.0 ± 0.2

0.6 ± 0.01^a

299.3 ± 15.8^b

0.9 ± 0.01

3.0 ± 0.3^a

2.3 ± 0.02^b

9.0 ± 0.9^a

36.0 ± 2.8^a