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Fig. 1 | Biotechnology for Biofuels

Fig. 1

From: Two bifunctional enzymes from the marine protist Thraustochytrium roseum: biochemical characterization of wax ester synthase/acyl-CoA:diacylglycerol acyltransferase activity catalyzing wax ester and triacylglycerol synthesis

Fig. 1

Sequence analysis of Thraustochytrium roseum WS/DGAT proteins TrWSD4 and TrWSD5. a Phylogenetic relationships among the deduced amino acid sequences of TrWSD4 and TrWSD5 proteins and the MGAT, DGAT1, DGAT2, and WS/DGAT family proteins (shaded in different colors) from a variety of organisms. The phylogenetic tree was constructed according to the Neighbor-Joining algorithm. GenBank accession numbers are shown by following the corresponding species name. Numbers at branch points are bootstrap percentages derived from 1000 replicates. b Predicted domains and motifs of TrWSD4 and TrWSD5. The WES_acyltransf (wax ester synthase-like Acyl-CoA acyltransferase) and DUF1298 (protein of unknown function) domains are shown by green and blue squares. The putative transmembrane domain of TrWSD5 and coiled coil domain of TrWSD4 are shown by red and purple squares. c The conserved HHXXXDG motif of WS/DGAT family members and the substitutions of serine (TrWSD4) and aspartate (TrWSD5) at the first residue of the motif are shown. Domain organization was predicted using HMMER ( and the sequence alignment was created with the BioEdit software

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