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Fig. 2 | Biotechnology for Biofuels

Fig. 2

From: Two bifunctional enzymes from the marine protist Thraustochytrium roseum: biochemical characterization of wax ester synthase/acyl-CoA:diacylglycerol acyltransferase activity catalyzing wax ester and triacylglycerol synthesis

Fig. 2

WS activity of recombinant TrWSD4 and TrWSD5 proteins purified from E. coli. The purified TrWSD4-6 × His (a) and TrWSD5-6 × His (b) were subjected to electrophoresis on a 12% Tris–HCl, SDS–polyacrylamide gel. The time-dependent CoA release catalyzed by TrWSD4 (c) and TrWSD5 (d) was monitored at A 412 for various protein concentrations: 0, 4, 8, 12, 16, and 20 μg. The concentrations of substrates palmitoyl-CoA (90 μM) and hexadecanol (100 μM) were constant. The time-dependent CoA release by 5 μg TrWSD4 (e) or 5 μg TrWSD5 (f) of varied palmitoyl-CoA concentrations: 0, 15, 30, 45, 60, 75, and 90 μM. The concentration of hexadecanol (100 μM) was constant. Reactions were performed at 37 °C in mixtures including 25 mM sodium phosphate buffer (pH 7.4) and 1 mg/mL DTNB

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