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Table 1 Kinetic parameters for TrWSD4-catalyzed esterification of hexadecanol and different acyl-CoAs

From: Two bifunctional enzymes from the marine protist Thraustochytrium roseum: biochemical characterization of wax ester synthase/acyl-CoA:diacylglycerol acyltransferase activity catalyzing wax ester and triacylglycerol synthesis

Substrate

K m (μM)

V max (nmol/min per mg)

k cat (s−1)

k cat/K m (M−1 s−1)

Octanoyl-CoA (C8:0)

29.93

14.76

1.85 × 10−2

6.19 × 102

Decanoyl-CoA (C10:0)

17.6

12.24

1.54 × 10−2

8.73 × 102

Lauroyl-CoA (C12:0)

0.14

16.08

2.02 × 10−2

1.46 × 105

Myristoyl-CoA (C14:0)

3.94

20.22

2.54 × 10−2

6.44 × 103

Palmitoyl-CoA (C16:0)

3.08

25.49

3.20 × 10−2

1.04 × 104

Stearoyl-CoA (C18:0)

19.15

30.23

3.79 × 10−2

1.98 × 103

Octadecatrienoyl-CoA (C18:3)

280.86

18.80

2.36 × 10−2

8.40 × 101

Arachidonoyl-CoA (C20:4)

297.17

11.82

1.48 × 10−2

4.99 × 101

Eicosapentaenoyl-CoA (C20:5)

131.16

9.05

1.14 × 10−2

8.66 × 101

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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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