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Table 1 Kinetic parameters for TrWSD4-catalyzed esterification of hexadecanol and different acyl-CoAs

From: Two bifunctional enzymes from the marine protist Thraustochytrium roseum: biochemical characterization of wax ester synthase/acyl-CoA:diacylglycerol acyltransferase activity catalyzing wax ester and triacylglycerol synthesis

Substrate K m (μM) V max (nmol/min per mg) k cat (s−1) k cat/K m (M−1 s−1)
Octanoyl-CoA (C8:0) 29.93 14.76 1.85 × 10−2 6.19 × 102
Decanoyl-CoA (C10:0) 17.6 12.24 1.54 × 10−2 8.73 × 102
Lauroyl-CoA (C12:0) 0.14 16.08 2.02 × 10−2 1.46 × 105
Myristoyl-CoA (C14:0) 3.94 20.22 2.54 × 10−2 6.44 × 103
Palmitoyl-CoA (C16:0) 3.08 25.49 3.20 × 10−2 1.04 × 104
Stearoyl-CoA (C18:0) 19.15 30.23 3.79 × 10−2 1.98 × 103
Octadecatrienoyl-CoA (C18:3) 280.86 18.80 2.36 × 10−2 8.40 × 101
Arachidonoyl-CoA (C20:4) 297.17 11.82 1.48 × 10−2 4.99 × 101
Eicosapentaenoyl-CoA (C20:5) 131.16 9.05 1.14 × 10−2 8.66 × 101