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Table 2 Kinetic parameters for TrWSD5-catalyzed esterification of hexadecanol and different acyl-CoAs

From: Two bifunctional enzymes from the marine protist Thraustochytrium roseum: biochemical characterization of wax ester synthase/acyl-CoA:diacylglycerol acyltransferase activity catalyzing wax ester and triacylglycerol synthesis

Substrate

K m (μM)

V max (nmol/min per mg)

k cat (s−1)

k cat/K m (M−1 s−1)

Octanoyl-CoA (C8:0)

12.25

63.92

1.02 × 10−1

8.33 × 103

Decanoyl-CoA (C10:0)

0.96

58.91

9.41 × 10−2

9.83 × 104

Lauroyl-CoA (C12:0)

25.93

151.9

2.43 × 10−1

9.37 × 103

Myristoyl-CoA (C14:0)

18.5

207.4

3.31 × 10−1

1.79 × 104

Palmitoyl-CoA (C16:0)

24.77

213.4

3.41 × 10−1

1.37 × 104

Stearoyl-CoA (C18:0)

75.87

378.5

6.04 × 10−1

7.96 × 103

Octadecatrienoyl-CoA (C18:3)

284.68

151.52

2.42 × 10−1

8.50 × 102

Arachidonoyl-CoA (C20:4)

226.35

103.09

1.65 × 10−1

7.29 × 102

Eicosapentaenoyl-CoA (C20:5)

106.2

311.7

4.98 × 10−1

4.69 × 103

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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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