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Table 2 Kinetic parameters for TrWSD5-catalyzed esterification of hexadecanol and different acyl-CoAs

From: Two bifunctional enzymes from the marine protist Thraustochytrium roseum: biochemical characterization of wax ester synthase/acyl-CoA:diacylglycerol acyltransferase activity catalyzing wax ester and triacylglycerol synthesis

Substrate K m (μM) V max (nmol/min per mg) k cat (s−1) k cat/K m (M−1 s−1)
Octanoyl-CoA (C8:0) 12.25 63.92 1.02 × 10−1 8.33 × 103
Decanoyl-CoA (C10:0) 0.96 58.91 9.41 × 10−2 9.83 × 104
Lauroyl-CoA (C12:0) 25.93 151.9 2.43 × 10−1 9.37 × 103
Myristoyl-CoA (C14:0) 18.5 207.4 3.31 × 10−1 1.79 × 104
Palmitoyl-CoA (C16:0) 24.77 213.4 3.41 × 10−1 1.37 × 104
Stearoyl-CoA (C18:0) 75.87 378.5 6.04 × 10−1 7.96 × 103
Octadecatrienoyl-CoA (C18:3) 284.68 151.52 2.42 × 10−1 8.50 × 102
Arachidonoyl-CoA (C20:4) 226.35 103.09 1.65 × 10−1 7.29 × 102
Eicosapentaenoyl-CoA (C20:5) 106.2 311.7 4.98 × 10−1 4.69 × 103