Fig. 3

The impact of co-expressing ScDGA1 and AtOLEO3 in Pt4 on growth and lipid accumulation in Pt4. Growth profiles for the transformants are based on cell concentration (a) and chlorophyll a content (b). The data points on these profiles represent a mean value from three independent studies, where in each study there were two biological replicates. Maximum lipid yield obtained from total lipids (black boxes) and TAGs (gray boxes) was also assessed for multigene and single gene expressing transformants (c). These yields were obtained from day 17; where transformants showed the highest yields. TAG productivities were calculated from maximum TAG yields (d). The values for these bar graphs represent mean values from three independent studies; in each study there were two biological replicates. Error bars were calculated from the standard deviation. p values were calculated from the student’s t test, where (asterisk) means p ≤ 0.05 and (double asterisk) means p ≤ 0.01. EV empty vector, TAG triacylglycerol, WT wild type. Raw data for growth profiles, lipid yields and TAG productivities can be obtained from Additional file 14