Fig. 6
From: A novel transcription factor specifically regulates GH11 xylanase genes in Trichoderma reesei
EMSAs of SxlR binding to the promoter regions of xylanase genes. a DNA binding of SxlR to the promoter regions of xyn1, xyn2, and xyn5. The amounts of purified SxlR binding domain (SxlR-B, μM) used were as indicated; ~10 ng of Cy5-labeled probe was added to each reaction. The shifts were verified to be specific by adding 100-fold excess of unlabeled specific (S) and non-specific (NS) competitor DNA. The SxlR-DNA complex is indicated by the arrow. b DNA binding of SxlR to the xyn3 and xyn4 promoter regions. We used three concentrations of SxlR-B: 0, 0.5, and 1 μM; ~10 ng of Cy5-labeled probe was added to each reaction