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Fig. 6 | Biotechnology for Biofuels

Fig. 6

From: Time-resolved transcriptome analysis and lipid pathway reconstruction of the oleaginous green microalga Monoraphidium neglectum reveal a model for triacylglycerol and lipid hyperaccumulation

Fig. 6

Schematic representation of the putative enzymatic steps of the central carbon metabolism in M. neglectum, including the transcriptional responses to N starvation (stages e−N and l−N) or N resupply (stage r+N). Enzymatic steps are represented by solid arrows and transport processes by dashed lines. The localization is drawn according to [32]. An expression pictogram plot for each enzymatic step is shown. It indicates the putative localization (C chloroplast, M mitochondrion, O other, S secretory pathway, NA not available due to truncation), transcript abundance at the reference time point N_0 (gray Roman numerals), and the transcriptional profile during the three stages of N availability. The three stages are represented by three color boxes, and individual time points by vertical color-coded bars in those boxes. The tag “NA” (not available) is added if the absolute transcript abundance (as FPKM) at that time point was less than 1.0. See Fig. 4 for a detailed description. The full annotations of the corresponding genes are given in Additional file 4. 1,3-BPA 1,3-bisphosphoglycerate, 2-PG 2-phosphoglycerate, 3-PG 3-phosphoglycerate, 6-PG 6-phosphogluconate, 6-PGL 6-phosphogluconolactonase, 6PGDH 6-phosphogluconate dehydrogenase, ACL ATP-citrate-lyase, ACS, acetyl-CoA synthetase, ADH bifunctional acetaldehyde-alcohol dehydrogenase, BASS2 sodium/pyruvate cotransporter BASS2 (bile acid-sodium symporter), CAH carbonic anhydrase, DHAP dihydroxyacetone phosphate, ENO enolase, FBA fructose-bisphosphate aldolase, FBP fructose-bisphosphate phosphatase, fruc fructose, G6PDH glucose-6-phosphate dehydrogenase, GA3P glyceraldehyde-3-phosphate, GAPDH glyceraldehyde-3-phosphate dehydrogenase, gluc glucose, GPDH, glycerol-3-phosphate dehydrogenase, HK hexokinase, MDH malate dehydrogenase, MME malic enzyme, PDC pyruvate decarboxylase, PDHC pyruvate dehydrogenase complex, PEP phosphoenolpyruvate, PEPC phosphoenolpyruvate carboxylase, PEPCK phosphoenolpyruvate carboxykinase, PFK phosphofructokinase, PFL pyruvate-formate-lyase, PFOR pyruvate-ferredoxin-oxidoreductase, PGI glucose-6-phosphate isomerase, PGK phosphoglycerate kinase, PGL 6-phosphogluconolactonase, PGM phosphoglycerate mutase, PK pyruvate kinase, PPT phosphoenolpyruvate transporter, PRK phosphoribulokinase, PYC pyruvate carboxylase, Ru5P ribulose-5-phosphate, RuBP ribulose-1,5-bisphosphate, TPI, triose phosphate isomerase, TPT triose phosphate transporter

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