Fig. 10
a Alignment of amino acid sequences of different XIs. RsXI-C1, novel XI cloned from the cDNA library of symbiotic protists of R. speratus; PiXI, XI of Piromyces sp. E2; CpXI, XI of C. phytofermentans; LlXI, XI of L. lactis. Amino acid sequence alignment was carried out using the genetic information analysis software GENETIX version 10. The shaded column indicates the point mutation site. b Xylose fermentation by recombinant yeast strains expressing mutated PiXI, CpXI, and LlXI. Xylose consumed after 72-h fermentation under microaerobic condition was shown. Xylose fermentation was carried out as described in "Methods". In all cases, initial cell density (absorbance at 600 nm, OD600) was 10.0. Error bars represent standard deviations of biological tetraplicates. Statistical significance was determined using Student’s t test. *P < 0.05, significant difference. n.s. no significant difference