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Table 1 Classification of thermotolerant genes and characterization of their Tn10-inserted mutants in Z. mobilis TISTR 548

From: Thermotolerant genes essential for survival at a critical high temperature in thermotolerant ethanologenic Zymomonas mobilis TISTR 548

Category Tn10-inserted gene Function Protein typea Growth at high temperature compared with that of parental strainb Sensitivity to ethanolc Effect of MgCl 2 d
38 °C 39 °C 39.5 °C 2.0% (v/v) 2.5% (v/v)
  (WT, TISTR548)    ++++ +++++ +++ ++++ ++++
General ZZ6_0707 Glucose sorbosone dehydrogenase S + + ++++ ++++
Metabolism (Group A) ZZ6_1376 5,10-methylenetetrahydrofolate reductase S ++++ ++++ + +++ +++ +++
Membrane ZZ6_1146 Glucosamine/fructose 6-phosphate aminotransferase M + + ++ ++ +++
Stabilization (Group B) ZZ6_0929 Glycosyltransferase group 1 S + ++++ + ++++
ZZ6_0923 Phospholipase D/transphosphatidylase M
ZZ6_1551 Squalene hopene cyclase (Shc) S + +++
ZZ6_1046 Tol/Pal system component TolQ M + + ++ ++
ZZ6_1043 Tol/Pal system component TolB S + + + ++++ ++++
ZZ6_1254 Protein export membrane protein SecD M ++ + ++
ZZ6_1477 Preprotein translocase subunit Tim44 M ++++ ++++
ZZ6_0158 Autotransporter secretion inner membrane protein TamB M + ++ ++ ++++
ZZ6_1210 Competence protein ComEC M + + +++
ZZ6_0840 Hypothetical transmembrane protein M ++++ +++ ++
ZZ6_0541 Hypothetical transmembrane protein M ++++ +++ + ++ ++ ++
Transporter (Group C) ZZ6_1289 Putative Fe2+/Mn2+ transporter M +++ +++
DNA repair (Group D) ZZ6_0616 DNA repair protein RadC S ++++ +++ + +++ +++
ZZ6_0934 Exonuclease VII (XseA) S +++ +++
ZZ6_0681 DNA repair protein RadA S + + +++ +++ ++
tRNA/rRNA modification (Group E) ZZ6_0023 tRNA/rRNA methyltransferase (SpoU) S +++ ++ ++ ++ ++ ++
Protein quality control (Group F) ZZ6_1659 Zn-dependent peptidase S ++++ +++ ++ ++++ ++++
ZZ6_0980 Serin protease DegP S +
Translation control (Group G) ZZ6_0702 ATP-dependent helicase HrpB S +
Cell division (Group H) ZZ6_0979 ParA/MinD-like ATPase S ++ ++
Transcriptional regulation (Group I) ZZ6_0019 Trp repressor-binding protein WrbA S +++ ++
Others ZZ6_0962 Pseudogene (S) + + ++++ ++++ ++
ZZ6_0861 Hypothetical protein S + + ++ ++
  1. aProtein type was described as described in "Methods" sections.”S” and “M” mean soluble protein and membrane protein, respectively
  2. bThe growth of representative of isolated mutants was compared to that of the parental strain on 3% YPD plates at 38, 39, and 39.5 °C. The number of “+” indicates the degree of cell growth at high temperature compared to that of the parental strain, while “−” indicates no growth
  3. cThe tolerance of representative of isolated mutants to ethanol was determined by comparison of growth on 3% YPD plates containing 2.0 and 2.5% (v/v) ethanol. The number of “+” indicates the degree of cell growth at 30 °C under the ethanol stress condition compared to that of the parental strain, while “−” indicates no growth
  4. dThe effect of MgCl2 on the growth of representative of isolated mutants was determined by comparison of growth in 3% YPD liquid medium containing 20 mM MgCl2 at 39.5 °C. The number of “+” indicates the following degree of cell growth compared to that of the growth in the absence of MgCl2: ++, P < 0.05; +++, P < 0.01; ++++, P < 0.001. “−” indicates no significant improvement of growth by the addition of MgCl2