Thermotolerant genes essential for survival at a critical high temperature in thermotolerant ethanologenic Zymomonas mobilis TISTR 548

Charoensuk, Kannikar; Sakurada, Tomoko; Tokiyama, Amina; Murata, Masayuki; Kosaka, Tomoyuki; Thanonkeo, Pornthap; Yamada, Mamoru

doi:10.1186/s13068-017-0891-0

Biotechnology for Biofuels and Bioproducts

Table 1 Classification of thermotolerant genes and characterization of their Tn10-inserted mutants in Z. mobilis TISTR 548

From: Thermotolerant genes essential for survival at a critical high temperature in thermotolerant ethanologenic Zymomonas mobilis TISTR 548

Category	Tn10-inserted gene	Function	Protein type^a	Growth at high temperature compared with that of parental strain^b			Sensitivity to ethanol^c		Effect of MgCl ^d₂
Category	Tn10-inserted gene	Function	Protein type^a	38 °C	39 °C	39.5 °C	2.0% (v/v)	2.5% (v/v)	Effect of MgCl ^d₂
	(WT, TISTR548)			++++	+++++	+++	++++	++++	−
General	ZZ6_0707	Glucose sorbosone dehydrogenase	S	+	+	−	++++	++++	−
Metabolism (Group A)	ZZ6_1376	5,10-methylenetetrahydrofolate reductase	S	++++	++++	+	+++	+++	+++
Membrane	ZZ6_1146	Glucosamine/fructose 6-phosphate aminotransferase	M	+	+	−	++	++	+++
Stabilization (Group B)	ZZ6_0929	Glycosyltransferase group 1	S	+	−	−	++++	+	++++
	ZZ6_0923	Phospholipase D/transphosphatidylase	M	−	−	−	–	−	–
	ZZ6_1551	Squalene hopene cyclase (Shc)	S	−	−	−	+	−	+++
	ZZ6_1046	Tol/Pal system component TolQ	M	+	+	−	++	++	−
	ZZ6_1043	Tol/Pal system component TolB	S	+	+	+	++++	++++	−
	ZZ6_1254	Protein export membrane protein SecD	M	−	−	−	++	+	++
	ZZ6_1477	Preprotein translocase subunit Tim44	M	−	−	−	++++	++++	−
	ZZ6_0158	Autotransporter secretion inner membrane protein TamB	M	+	−	−	++	++	++++
	ZZ6_1210	Competence protein ComEC	M	−	−	−	+	+	+++
	ZZ6_0840	Hypothetical transmembrane protein	M	−	−	−	++++	+++	++
	ZZ6_0541	Hypothetical transmembrane protein	M	++++	+++	+	++	++	++
Transporter (Group C)	ZZ6_1289	Putative Fe²⁺/Mn²⁺ transporter	M	−	−	−	+++	+++	−
DNA repair (Group D)	ZZ6_0616	DNA repair protein RadC	S	++++	+++	+	+++	+++	−
	ZZ6_0934	Exonuclease VII (XseA)	S	−	−	−	+++	+++	−
	ZZ6_0681	DNA repair protein RadA	S	+	+	−	+++	+++	++
tRNA/rRNA modification (Group E)	ZZ6_0023	tRNA/rRNA methyltransferase (SpoU)	S	+++	++	++	++	++	++
Protein quality control (Group F)	ZZ6_1659	Zn-dependent peptidase	S	++++	+++	++	++++	++++	−
Protein quality control (Group F)	ZZ6_0980	Serin protease DegP	S	−	−	−	−	−	+
Translation control (Group G)	ZZ6_0702	ATP-dependent helicase HrpB	S	−	−	−	+	−	−
Cell division (Group H)	ZZ6_0979	ParA/MinD-like ATPase	S	−	−	−	++	++	−
Transcriptional regulation (Group I)	ZZ6_0019	Trp repressor-binding protein WrbA	S	−	−	−	+++	++	−
Others	ZZ6_0962	Pseudogene	(S)	+	+	−	++++	++++	++
Others	ZZ6_0861	Hypothetical protein	S	+	+	−	++	++	−

^aProtein type was described as described in "Methods" sections.”S” and “M” mean soluble protein and membrane protein, respectively
^bThe growth of representative of isolated mutants was compared to that of the parental strain on 3% YPD plates at 38, 39, and 39.5 °C. The number of “+” indicates the degree of cell growth at high temperature compared to that of the parental strain, while “−” indicates no growth
^cThe tolerance of representative of isolated mutants to ethanol was determined by comparison of growth on 3% YPD plates containing 2.0 and 2.5% (v/v) ethanol. The number of “+” indicates the degree of cell growth at 30 °C under the ethanol stress condition compared to that of the parental strain, while “−” indicates no growth
^dThe effect of MgCl₂ on the growth of representative of isolated mutants was determined by comparison of growth in 3% YPD liquid medium containing 20 mM MgCl₂ at 39.5 °C. The number of “+” indicates the following degree of cell growth compared to that of the growth in the absence of MgCl₂: ++, P < 0.05; +++, P < 0.01; ++++, P < 0.001. “−” indicates no significant improvement of growth by the addition of MgCl₂

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