Fig. 6

Multifeed SSCF at constant temperature or with temperature reduction. a Cell viability (% of CFU/total cell counts) and accumulated WIS content (indicating substrate feeding); and b concentration of glucose, xylose, and ethanol in 25% (w/w) WIS multifeed SSCF of material M2, with the KE-Flow strain, at a constant temperature of 35 °C, or at 35 °C for 24 h, and then at 30 °C. Arrows between the panels indicate cell additions. Details of the substrate and cell feeding can be found in Additional file 1: Table S1