Fig. 2

Phylogenetic relationship between MA-13 and other B. coagulans strains based on 16S rRNA sequences. The evolutionary history was inferred by using the Maximum Likelihood method based on the Tamura–Nei model [35]. The tree with the highest log likelihood (−1664.1025) is shown. The percentage of trees in which the associated taxa clustered together is shown next to the branches. Initial tree(s) for the heuristic search were obtained by applying the Neighbour-Joining method to a matrix of pairwise distances estimated using the Maximum Composite Likelihood approach. The tree is drawn to scale, with branch lengths measured in the number of substitutions per site (0.01). The analysis involved 24 nucleotide sequences. All positions containing gaps and missing data were eliminated. There was a total of 745 positions in the final dataset. Evolutionary analyses were conducted in MEGA6, using the 16S sequence from Bacillus circulans 5S5 as an outgroup to root the tree. For all strains, the GenBank accession number is reported