Fig. 6From: Genome sequencing and transcriptome analysis of Trichoderma reesei QM9978 strain reveals a distal chromosome translocation to be responsible for loss of vib1 expression and loss of cellulase inductionVIB1 is required for cellulase production and signaling. Expression levels for the cellulase gene cbh1 a, the transcription factor vib1 b, and the main positive regulator xyr1 c were assessed in −/+vib1 strains versus WT after a shift from glycerol to 1% (v/w) lactose. Gene expression levels were measured by relative quantitative PCR using tef1 and sar1 as a control and normalized to expression level at time point zero (24 h growth on glycerol) for cbh1 and xyr1 or to expression level in QM9978 for vib1. Samples were taken 5 h (black), 16 h (light gray), and 24 h (dark gray) after replacement. Mean values ± standard error of triplicate data from two biological replicates are shown. ND not detectedBack to article page