Fig. 6

VIB1 is required for cellulase production and signaling. Expression levels for the cellulase gene cbh1 a, the transcription factor vib1 b, and the main positive regulator xyr1 c were assessed in −/+vib1 strains versus WT after a shift from glycerol to 1% (v/w) lactose. Gene expression levels were measured by relative quantitative PCR using tef1 and sar1 as a control and normalized to expression level at time point zero (24 h growth on glycerol) for cbh1 and xyr1 or to expression level in QM9978 for vib1. Samples were taken 5 h (black), 16 h (light gray), and 24 h (dark gray) after replacement. Mean values ± standard error of triplicate data from two biological replicates are shown. ND not detected