Fig. 3

Phylogenetic tree of B. cellulosolvens cohesin modules. Cohesin modules were selected according to bioinformatic analysis, and the sequences were chosen individually to include only the cohesin sequence itself, without linkers or other scaffoldin modules. The final digit of each name represents the number of the specific cohesin from the N terminus in a multivalent scaffoldin. The phylogenetic tree was generated by iTOL according to the “One Click” Phylogeny analysis tool and aligned manually by BioEdit. Colors represent cohesin-type classification: red lines refer to type I cohesins; blue line to type II; pink line represent Group R cohesins, and green lines type III. Within the tree we used three sequences of known previously defined cohesins (from each type: I, II, and III) from other bacteria to serve as standards or markers for comparison (the names of the cohesins colored according to the type). Red stars represent cohesins that were expressed and experimentally tested in the present work for dockerin specificity. Colors in the inner (complete) circle facilitate visual identification of the cohesin types (see key, above right). Colors in the outer (fragmented) circle represent the cluster of the designated cohesins (see key, below right, and Fig. 2). Numbers on the branches represent bootstrap values