The yeast Geotrichum candidum encodes functional lytic polysaccharide monooxygenases

Table 1 Production and activity details of AA9 LPMO used in this study

Protein	Modularity	Predicted N-glycosylation sites	Signal peptide	Production yield (g/L)	Specific activity (U/g)^a	Total activity (U/L)	Competitive assay (% residual activity)
Protein	Modularity	Predicted N-glycosylation sites	Signal peptide	Production yield (g/L)	Specific activity (U/g)^a	Total activity (U/L)	PASC (0.1%)	Xyloglucan (0.1%)
GcLPMO9A	1-20[SP]-21-243[AA9]-244-288[Cterm]	6	α-factor	0.062	0.174	0.011	ND	ND
GcLPMO9A	1-20[SP]-21-243[AA9]-244-288[Cterm]	6	Native	0.110	0.286	0.031	33.9 ± 5.4	16.3 ± 0.4
GcLPMO9B	1-18[SP]-19-244[AA9]-245-359[Cterm]	10	α-factor	0.113	0.011	0.001	ND	ND
GcLPMO9B	1-18[SP]-19-244[AA9]-245-359[Cterm]	10	Native	0.194	1.012	0.196	24.0 ± 0.6	15.1 ± 3.8
GcLPMO9C	1-18[SP]-19-242[AA9]-243-530[Cterm]	12	α-factor	0.150	0.020	0.003	ND	ND
GcLPMO9C	1-18[SP]-19-242[AA9]-243-530[Cterm]	12	Native	0.072	0.474	0.034	ND	ND

SP signal peptide, ND not determined, nd not detected, AA9 AA9 catalytic module
^aSpecific activity was determined using amplex red assay. N-glycosylation sites were predicted using the Hirst group prediction server [40]

ISSN: 2731-3654