Fig. 5
Optimized production of 11α-hydroxyprogesterone and ethanol using AH22:p11α-SHAoch yeast grown in batch culture with YM+His and 300 μM progesterone. Three shaker speeds, 30 (◆), 100 (□), 150 (●), and 200 (◊) rpm, were used. The arrow indicates the time point (t 10 h) at which the speed of AH22:p11α-SHAoch cultures maintained at 200 rpm (optimal for progesterone bioconversion) a was either held at 200 rpm or decreased to 150, 100, or 30 rpm during the ethanol fermentation step (b). Mean values of three replicates along with associated standard deviations are shown