Co-production of 11α-hydroxyprogesterone and ethanol using recombinant yeast expressing fungal steroid hydroxylases

Table 1 GC–MS parameters and diagnostic ions for progesterone (Pg) substrate and hydroxylated (hydroxyPg) products

Peak	Gas chromatography–mass spectrometry: steroid identification				Steroid: % total (t ₄₈ h)
Peak	Steroid	RT (min)	[M]⁺	Fragment ions m/z (relative intensity)	AH22:p425TEF^Ctrl	AH22:p11α-SH^Aoch	AH22:pCYP509C12
a	Progesterone (Pg)	21.04	314	43 (999) 124 (887) 314 (701) 272 (430) 229 (362) 91 (329) 41 (314) 79 (313) 22 (238) 67 (234)	100	9.90 (±1.2)	45.54 (±3.1)
b	Pg + TMS (×1)	20.36	386	386 (999) 73 (820) 43 (365) 387 (311) 371 (145) 75 (141) 209 (132) 208 (118) 91 (101) 196 (97)	100	9.90 (±1.2)	45.54 (±3.1)
c	11α-hydroxyPg + TMS (×2)	21.61	474	73 (999) 474 (559) 43 (230) 192 (227) 475 (219) 75 (198) 193 (126) 208 (85) 74 (84) 476 (80)	0	90.10 (±1.6)	47.61 (±1.7)
d	11α-hydroxyPg + TMS (×1)	23.29	402	73 (999) 43 (825) 75 (395) 402 (347) 91 (310) 312 (277) 197 (260) 143 (253) 169 (233) 105 (223)	0	90.10 (±1.6)	47.61 (±1.7)
e	6β-hydroxyPg + TMS (×1)	21.40	402	73 (999) 43 (906) 346 (712) 75 (688) 91 (386) 387 (347) 79 (295) 93 (262) 402 (240) 119 (226)	0	0	6.85 (±1.7)
Maximum % bioconversion (ΣhydroxyPg)					0.00	91.70	57.86

Steroid (%) abundances are mean values from three replicates with associated standard deviations
TMS trimethylsilyl derivatized, RT retention time, [M] ⁺ mass ion

ISSN: 2731-3654