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Table 3 Bioconversion of progesterone to 11α-hydroxyprogesterone with AH22:11α-SHAoch+K

From: Co-production of 11α-hydroxyprogesterone and ethanol using recombinant yeast expressing fungal steroid hydroxylases

Grass juice: yeast minimal

Inoculum (cells/mL)

Temperature (°C)

% product formed (µmol/L)

1:0

1 × 107

30

47 (448)

1:0

1 × 107

24

54 (515)

1:0

1 × 105

30

44 (420)

1:0

1 × 105

24

58 (553)

1:1

1 × 107

30

44 (420)

1:1

1 × 107

24

58 (553)

1:1

1 × 105

30

44 (420)

1:1

1 × 105

24

62 (591)

1:3

1 × 107

30

42 (401)

1:3

1 × 107

24

59 (563)

1:3

1 × 105

30

50 (477)

1:3

1 × 105

24

60 (572)

  1. Progesterone bioconversion rates obtained using the integrated clone AH22:11α-SHAoch+K with 0.3 mg/mL (954 µM) progesterone in growth media after 48 h
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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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