Table 2 Substrate specificities of Lac and VP purified from P. vitreus
Substrate | Wavelength (nm) | Molar extinction coefficient ε (L mol cm−1) | K m (mol L−1) | V max (μmol L−1 min−1) | K cat (s−1) | K cat/K m (s−1 mM−1) | ||||
|---|---|---|---|---|---|---|---|---|---|---|
Lac | VP | Lac | VP | Lac | VP | Lac | VP | |||
ABTS | 420 | 36,000 | 1.3 × 10−5 | 3.5 × 10−5 | 16.8 | 32.3 | 22.8 | 17.2 | 1791.9 | 487.5 |
2,6-DMP | 470 | 49,600 | 2.8 × 10−4 | 4.4 × 10−5 | 5.2 | 66.7 | 7.1 | 35.5 | 25.1 | 804.2 |
Guaiacol | 465 | 12,100 | 9.0 × 10−4 | 1.3 × 10−4 | 1.7 | 85.5 | 2.2 | 45.5 | 2.5 | 347.1 |
MnSO4 | 270 | 11,590 | – | 5.4 × 10−5 | – | 18.2 | – | 9.7 | – | 179.1 |
H2O2 | 270 | 11,590 | – | 4.4 × 10−5 | – | 39.7 | – | 21.1 | – | 475.4 |
RB5 | 598 | 59,800 | – | 1.4 × 10−5 | – | 14.5 | – | 7.7 | – | 543.2 |
VA | 310 | 4700 | – | 2.1 × 10−4 | – | 23.1 | – | 12.3 | – | 57.7 |