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Fig. 1 | Biotechnology for Biofuels

Fig. 1

From: How does cellulosome composition influence deconstruction of lignocellulosic substrates in Clostridium (Ruminiclostridium) thermocellum DSM 1313?

Fig. 1

Specific activities of the various cellulosome fractions: cellulosomes derived from glucose-, cellobiose (CB)-, microcrystalline cellulose (MCC)-, alkaline-pretreated switchgrass (alSG)-, alkaline-pretreated corn stover (alCS)-, and dilute acid-pretreated corn stover (acCS)-based growth media were applied on the different substrates and released soluble sugars were measured by the DNS method. The percent content of relevant enzymes (cellulosomal subunits and soluble carbohydrate-active enzymes) in each sample was calculated using MS, and specific activities were calculated [µM reducing ends µg protein−1 min−1]. a Cellulosomes (20 µg) were applied to 70 mg MCC for 19 h; b cellulosomes (60 µg) were applied to 50 mg alSG for 16 h; c cellulosomes (3 µg) were applied to 50 mg alCS for 15 h. d Cellulosomes (50 µg) were applied to 50 mg acCS for 18 h. Cellulosomes and biomass dosages were determined in preliminary calibration assays in order to work in the near-linear range of the reaction (Additional file 2: Figure S2). All assays were performed at a final volume of 1 mL, at 70 °C, with the addition of 0.33 mg/mL equivalent of Thermoanaerobacter brockii β-glucosidase (CglT) in order to prevent feedback inhibition

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