Fig. 5

Cellulosomal subunit composition. Subunit compositions of the different cellulosomes were analyzed by label-free LC–MS/MS mass spectrometry. The intensities were normalized by intensity-based absolute quantification (iBAQ) method. The resultant iBAQ intensities of type I dockerin-containing subunits were divided by the iBAQ intensity of ScaA in each sample, thereby generating a relative abundance index. Average and standard deviations of duplicate samples of CB- and MCC-derived cellulosomes and triplicates of glucose-, alSG-, alCS-, and acCS-derived cellulosomes were analyzed. The 20 most abundant subunits in each sample were rated by a colored scale. Gene ID and CAZY annotation of the subunits are mentioned in the table too. GH, glycoside hydrolase; CBM, carbohydrate-binding module; CE, carbohydrate esterase