Fig. 4

Structural comparison of McCut and other cutinases. a Superposition of McCut on other cutinases is shown by ribbon diagram, with ribbons colored according to each enzyme: McCut in green, AoCut (PDB code: 3GBS) in blue, FsCut (PDB code: 1CEX) in yellow, CLE (PDB code: 2CZQ) in cyan, and G. cingulata cutinase (PDB code: 3DCN) in orange. b The unique loop is further shown as a cartoon in the comparison structure. The difference of Ser34 in McCut leads to the space steric hindrance. c The gatekeeper residues Leu73 and Leu176 in McCut, and Leu87 and Val190 in AoCut are shown