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Table 1 Effect of organic solvents and surfactants on the enzyme activity of McCut

From: High-level expression and characterization of a novel cutinase from Malbranchea cinnamomea suitable for butyl butyrate production

Solvent

Specific activity (U/mg)

Relative activity (%)

Organic solvent (30%)

 Control

1115.4 ± 11.5

100

 Methanol

891.6 ± 4.6a

79.9

 Ethanol

811.6 ± 43.9a

72.8

 Isopropanol

908.8 ± 40.4a

81.5

 Butanol

656.4 ± 16.2a

58.8

 Acetic acid

649.1 ± 17.3a

58.2

 Butyric acid

398.3 ± 18.5a

35.7

 n-Hexane

1090.1 ± 35.8

97.7

 Cyclohexane

1426.6 ± 32.8a

127.9

 n-Heptane

1465.0 ± 11.6a

131.3

 Octane

1601.1 ± 8.2a

143.5

 Isooctane

1089.3 ± 39.3

97.7

 Acetone

983.1 ± 6.9a

88.1

 Dimethyl sulfoxide

902.3 ± 33.5a

80.9

 Acetonitrile

1038.1 ± 7.7a

93.1

Surfactant (5%)

 Tween 20

1316.1 ± 39.3a

118.0

 Tween 40

1557.9 ± 55.4a

139.7

 Tween 60

1555.7 ± 81.4a

139.5

 Tween 80

1582.4 ± 20.8a

141.9

 Triton X-100

2120.9 ± 40.8a

190.1

 SDS

1468.6 ± 44.6a

131.7

  1. The enzyme was incubated with various organic solvents and surfactants at 50 °C for 1 h in 50 mM Tris–HCl pH 8.0, and the residual activity was measured according to the standard method. All data are mean values ± standard deviations of triplicate measurements
  2. aThe difference is significant compared with the control (P < 0.05)