Fig. 5

Saccharification of PCS with enzyme loading from 20 to 80 μL by T. reesei RUT-C30 and SEU-7 using the equal culture supernatants of RUT-C30 and SEU-7 on 2% cellulose or 3% lactose, respectively. Saccharification of corn stalk pretreated with EDA (a) or alkali (b) by crude enzyme solution produced from strain SEU-7 and RUT-C30 grown on lactose or cellulose, which was designated SEU-7-lac, SEU-7-cel, C30-lac, and C30-cel, respectively. The crude enzyme solution prepared from fed-batch culture of SEU-7 on 3% lactose was named as SEU-7-lac-fed. The error bars indicate the standard deviations of three biological replicates