Fig. 2

Subcellular localization of EGFP in transgenic C. vulgaris cells. a Schematic diagram of binary plasmid pBI-tpEGFP. RB right border, LB left border, Nos-Pro nopaline synthase promoter, Nos-Ter nopaline synthase terminator, NPTII neomycin phosphotransferase, 35S-Pro CaMV35S promoter, cTP chloroplast transit peptide, EGFP enhanced green fluorescent protein. b EGFP expression in transgenic C. vulgaris cells. EGFP fluorescence, corresponding chloroplast autofluorescence, and bright field of algal cells are present on the left, middle, and right columns, respectively. The first line showed that no green fluorescence of EGFP was found in wild-type (WT) cells using a narrow band filter. As shown in the second line, given that red fluorescence image indicated the location of the chloroplast, green fluorescence from EGFP was detected in the chloroplast of transformed cells into which the EGFP gene fused with cTP sequence was introduced. The third line showed that green fluorescence from EGFP was detected in the cytoplasm of transformed cells introduced with the EGFP gene omitting the above cTP sequence. Bars, 10 µm